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split-luciferase receptor assay glosensor camp assay  (Promega)

 
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    Structured Review

    Promega split-luciferase receptor assay glosensor camp assay
    Concentration-dependent and GPR39-dependent <t>cAMP</t> (Gs) responses, as measured by the <t>Glosensor</t> assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.
    Split Luciferase Receptor Assay Glosensor Camp Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/split-luciferase+receptor+assay+glosensor+camp+assay/pmc05118639-219-11-14?v=Promega
    Average 90 stars, based on 1 article reviews
    split-luciferase receptor assay glosensor camp assay - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc "

    Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc

    Journal: Molecular Pharmacology

    doi: 10.1124/mol.116.106112

    Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.
    Figure Legend Snippet: Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.

    Techniques Used: Concentration Assay

    Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.
    Figure Legend Snippet: Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.

    Techniques Used: Concentration Assay, Mutagenesis



    Similar Products

    split-luciferase receptor assay glosensor camp assay  (Promega)
    90
    Promega split-luciferase receptor assay glosensor camp assay
    Concentration-dependent and GPR39-dependent <t>cAMP</t> (Gs) responses, as measured by the <t>Glosensor</t> assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.
    Split Luciferase Receptor Assay Glosensor Camp Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/split-luciferase+receptor+assay+glosensor+camp+assay/pmc05118639-219-11-14?v=Promega
    Average 90 stars, based on 1 article reviews
    split-luciferase receptor assay glosensor camp assay - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.

    Journal: Molecular Pharmacology

    Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc

    doi: 10.1124/mol.116.106112

    Figure Lengend Snippet: Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.

    Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (GloSensor cAMP assay; Promega, Madison, WI).

    Techniques: Concentration Assay

    Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.

    Journal: Molecular Pharmacology

    Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc

    doi: 10.1124/mol.116.106112

    Figure Lengend Snippet: Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.

    Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (GloSensor cAMP assay; Promega, Madison, WI).

    Techniques: Concentration Assay, Mutagenesis